My dear husband is so so busy these days that I pop in at the lab when everybody else is gone to keep him company. But I like to help if I can, and this is what I have been doing lately for him to use in his experiments. Bear with me, because this is long...
You secure the dish in the lathe:
![](https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgc8FfxOH4KrfLXx5LUS5aQ7nC1iJcCaiCWGVFivgDovYMQ8aUjOY9xrNhmuw_UZZ9RqY6cw7Ixfj6A1SRl5fvJrJ8J8EQMekRzPIJDvNw2NdDgd07uA-0pHD0kYwvHOPVEHgP4cAneI1rE/s320/P1060772-2.jpg)
![](https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjDy_cb59Ztats7_hShQR07ji8VUNt2zfFLkfVN6tNTxa-SY-y85VnRcEqTKOYDQO0eNXkivQfVGZdoQCN5vCHc84eBE4zS8xarE4tg0S88gnbQ3DFLqibTe2gcf6W8QfZAxxebnRGuE17Q/s320/P1060774-2.jpg)
When you have made holes in 20-25 dishes, you clean them up, scratching the rests of the plastic that have not come out. If you skip this step, cells get poisoned by the scraps and then they die :(
![](https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi7gZY1QyaGFjXVKZmVglI1xKtYlNKNTR4AEGjuedGoajckXhFmXquqGZf9Ari7GX9dSB_y_MVFB_uw87dC2HlDhn8sRpJ-Ne2cvzeD5HyJGzWyZ9nHEjCwmer1-tjA_1B2og8YqpQL11rR/s320/P1060768-2.jpg)
![](https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhYGWQwbV9nraDnrgFte_sWi0gLtQByJRxkmAD68JN0R_z7LLnLKlvyuph_F2Vnrw9GEOB5qEd3QOx9QwxYPB9J9ysfYiwJWRnJTOiLRs0lhqIFJeMyZ4jL7w7NXOkP-nbqv2WERC50KiKt/s320/P1060759-2.jpg)
![](https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgIAudUaRCMzQZnoI7ixhEWC0PvqSMRm6nq31ndaJCzvRC7BrRNo5La-H7qnaldsdFnfU9MYUNC_3oljnZvg1bUhDpRKIfTUkrdrCVp_TFYcnQf3sOMO0mmAk-jdGFGd_fQ6yuWDjQLKBiU/s320/P1060760-2.jpg)
Then you take a glass cover and stick it to the glue:
![](https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhZi3ghYtY4zPu5MnKcT89zG6J8q8Orr9iLCjMDMqdgGAB74DaO4pJutNLu2-nUR2q_WqR9S0w2h70KrfJerMa8MnxTmlWZa1R5T5dpbW05l4mk02X0HOSmEC4gddQ4tqY7gq3IO_dxJkEh/s320/P1060770-2.jpg)
You have to squeeze out the air bubbles:
![](https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjouWGz6K4Xds9KXO-iUGGw0gG73Djjap6Llt7XsSby16yIoWiwztbZFfaZO7JAcQBG0Wpnb377eEJ8Pizv2gyud0qCDg_uXqfzyq-iJnoFn5BHUiF-5iJnzOl56BzidoduFE9jh77YWGzE/s320/P1060771-2.jpg)
![](https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEiby23Bfby7EM13Og9JEGljdnDAOOq6Yhx4NS0OT5X2gUvUkSwpQJ3SUTBxT2ABTUGYFvZ4BXSOLgSLoXQyE5ZuhxeqUvzvZBquhAqMRoWCBNGtFqsqRgFqqfr8K-ry8OsIkku3SqTDafS_/s320/P1060782-2.jpg)
Oh my, what a nerd I am :)